A simplified method for the identification of urinary histidine(Kapeller-Adler reaction), tryptophan and the color reactions for the derivative of its metabolite indol (Ashitaka reaction) was tested.
The test was performed using, a urine specimen from 120 normal pregnant women, 52 on-pregnant women, 40 male adults 8 girls and boys, 30 puerpera, and 39 pregnancy abnormalities such as preeclampsia, ectopic gestation, abortion and, premature delivery.
Results
I) Of 120 normal pregnant urine tests, there were 72 positive responses of histidine and 94 positive responses, of tryptophan:
Accuracies Histidine 60.0 %
Tryptophan 78.5 %
In the first trimester of pregnancy the accuracies of 30 % and 70 % were obtained for histidine and tryptophan,respectively.
The increased excretions of urinary histidine were not found before the third month of pregnancy and cannot, therefore, be used as an aid in the early diagnosis of -pregnancy. It was also experimentally shown that tryptophan was more closely related to pregnancy.
2) It was interesting that all cases of preeclampsia showed , negative responses for histidine but 50 % positive responses for tryptophan.
3) The histidine and tryptophan tests in the urine of pregnancy abnormalities, showed low, positive responses (about 50 %) and pregnancy abnormality reaction was positive in about 50 percent.
4) In the test of puerperal women en urine, the response became gradually negative from the second postpartum day and at least almost completely negative within the seventh postpartum day.
5) There was no detectable difference in histidine and tryptophan reaction between non-pregnant womem and men.
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